Journal: Neuropharmacology

Article Title: Vortioxetine attenuates the effects of early-life stress on depression-like behaviors and monoamine transporters in female mice.

doi: 10.1016/j.neuropharm.2021.108468

Figure Lengend Snippet: Fig. 1. Sex-specific changes of depression-like behaviors induced by ELS in adult mice. (A) The experimental time line of ELS exposure, depression-like behav ioral tests, and immunohistochemistry. (B–D) Schematic diagram of behavioral tests and effects of ELS on depression-like be haviors: sucrose preference test (B), tail suspension test (C), and forced swim test (D) in adult male and female mice. CT, control; ES, Early-life stress; FST, forced swim test; IHC, immunohistochemistry; PND, postnatal day; SPT, sucrose preference test; TST, tail suspension test. & p < 0.05, the stress × sex interaction; ##p < 0.01, the main effect of sex; *p < 0.05, **p < 0.01, ***p < 0.001, Tukey’s post hoc tests.

Article Snippet: The following primary antibodies were used for immunostaining: rabbit anti-DAT (1:500, Cat. No. 284003, Synaptic systems, Germany), mouse anti-NET (1:1,000, Cat. No. X. Liu et al. Neuropharmacology 186 (2021) 108468 260011, Synaptic systems, Germany), rabbit anti-SERT (1:1,000, Cat. No. 340003, Synaptic systems, Germany), and rabbit anti-VMAT2 (1:200, Cat. No. 138313, Synaptic systems, Germany).

Techniques: Immunohistochemistry, Suspension, Control

Journal: Investigative Ophthalmology & Visual Science

Article Title: Normalization of Wound Healing and Diabetic Markers in Organ Cultured Human Diabetic Corneas by Adenoviral Delivery of c-Met Gene

doi: 10.1167/iovs.09-4569

Figure Lengend Snippet: Antibodies

Article Snippet: The following gene-specific assay kits were used as per ABI: c-met proto-oncogene assay ID Hs00179845_m1 (GenBank RefSeq {"type":"entrez-nucleotide","attrs":{"text":"NM_001127500.1","term_id":"188595715","term_text":"NM_001127500.1"}} NM_001127500.1 , exon boundary 10-11; assay location, 2605; amplicon size, 81 bp) and β 2 -microglobulin assay ID Hs99999907_m1 (GenBank RefSeq {"type":"entrez-nucleotide","attrs":{"text":"NM_004048.2","term_id":"37704380","term_text":"NM_004048.2"}} NM_004048.2 , exon boundary 2-3; assay location, 413; amplicon size, 75 bp) ( www.ncbi.nlm.nih.gov/locuslink/refseq/ RefSeq is provided in the public domain by the National Center for Biotechnology Information, Bethesda, MD).

Techniques: Western Blot, Transduction, Immunohistochemistry

Journal: Investigative Ophthalmology & Visual Science

Article Title: Normalization of Wound Healing and Diabetic Markers in Organ Cultured Human Diabetic Corneas by Adenoviral Delivery of c-Met Gene

doi: 10.1167/iovs.09-4569

Figure Lengend Snippet: rAV-cmet transduction into organ cultured human diabetic corneas and U87MG cells led to c-met overexpression. (A) Immunoblot analysis of total protein from corneas transduced with rAV-cmet and rAV-vector probed with rabbit pAb sc-161 (left), and from U87MG cell lysates (right). Arrows: 170-kDa full-length c-met, 140- to 145–kDa processed chain, and 55- to 60-kDa cell-associated fragment. Note that, in glioma cells, transduced c-met gets processed and mainly a 145-kDa chain is detected, whereas in the corneas, a full-length protein is present together with a prominent smaller band, apparently corresponding to a cell-associated kinase fragment. β-Actin was used to normalize protein loading. M, markers in kilodaltons. (B) Quantitative RT-PCR. On c-met gene transduction, its mRNA level increased approximately threefold in the epithelium, but stromal level did not change. Ratios to vector control are presented. β2-microglobulin was used as the housekeeping gene.

Article Snippet: The following gene-specific assay kits were used as per ABI: c-met proto-oncogene assay ID Hs00179845_m1 (GenBank RefSeq {"type":"entrez-nucleotide","attrs":{"text":"NM_001127500.1","term_id":"188595715","term_text":"NM_001127500.1"}} NM_001127500.1 , exon boundary 10-11; assay location, 2605; amplicon size, 81 bp) and β 2 -microglobulin assay ID Hs99999907_m1 (GenBank RefSeq {"type":"entrez-nucleotide","attrs":{"text":"NM_004048.2","term_id":"37704380","term_text":"NM_004048.2"}} NM_004048.2 , exon boundary 2-3; assay location, 413; amplicon size, 75 bp) ( www.ncbi.nlm.nih.gov/locuslink/refseq/ RefSeq is provided in the public domain by the National Center for Biotechnology Information, Bethesda, MD).

Techniques: Transduction, Cell Culture, Over Expression, Western Blot, Plasmid Preparation, Quantitative RT-PCR

Journal: Investigative Ophthalmology & Visual Science

Article Title: Normalization of Wound Healing and Diabetic Markers in Organ Cultured Human Diabetic Corneas by Adenoviral Delivery of c-Met Gene

doi: 10.1167/iovs.09-4569

Figure Lengend Snippet: Transduction of c-met to organ cultured human diabetic corneas caused its increased expression and phosphorylation. Left: rAV-vector transduction; right: rAV-cmet transduction. In corneas transduced with rAV-cmet, there was increased epithelial staining for total c-met (revealed with a C-terminal rabbit pAb sc-10), tyrosine-phosphorylated (PY) c-met, and extracellular (EX) c-met. Indirect immunofluorescence of corneal sections. Sections of wounded and healed corneas are shown; the same exposure time was used for each pair of fellow corneas. e, epithelium; s, stroma.

Article Snippet: The following gene-specific assay kits were used as per ABI: c-met proto-oncogene assay ID Hs00179845_m1 (GenBank RefSeq {"type":"entrez-nucleotide","attrs":{"text":"NM_001127500.1","term_id":"188595715","term_text":"NM_001127500.1"}} NM_001127500.1 , exon boundary 10-11; assay location, 2605; amplicon size, 81 bp) and β 2 -microglobulin assay ID Hs99999907_m1 (GenBank RefSeq {"type":"entrez-nucleotide","attrs":{"text":"NM_004048.2","term_id":"37704380","term_text":"NM_004048.2"}} NM_004048.2 , exon boundary 2-3; assay location, 413; amplicon size, 75 bp) ( www.ncbi.nlm.nih.gov/locuslink/refseq/ RefSeq is provided in the public domain by the National Center for Biotechnology Information, Bethesda, MD).

Techniques: Transduction, Cell Culture, Expressing, Plasmid Preparation, Staining, Immunofluorescence

Journal: Investigative Ophthalmology & Visual Science

Article Title: Normalization of Wound Healing and Diabetic Markers in Organ Cultured Human Diabetic Corneas by Adenoviral Delivery of c-Met Gene

doi: 10.1167/iovs.09-4569

Figure Lengend Snippet: c-Met transduction to organ cultured human diabetic corneas normalized expression patterns of select diabetic markers. Staining for integrin α3β1 became more homogeneous, and its intensity increased (top row). Staining for the BM component laminin-γ1 chain appeared after c-met transduction (second row). Staining for BM nidogen-1 and -2 (double labeling is shown in the two bottom rows) remained interrupted on vector transduction but became homogeneous after c-met transduction, similar to normal corneas. Indirect immunofluorescence of corneal sections. e, epithelium; s, stroma.

Article Snippet: The following gene-specific assay kits were used as per ABI: c-met proto-oncogene assay ID Hs00179845_m1 (GenBank RefSeq {"type":"entrez-nucleotide","attrs":{"text":"NM_001127500.1","term_id":"188595715","term_text":"NM_001127500.1"}} NM_001127500.1 , exon boundary 10-11; assay location, 2605; amplicon size, 81 bp) and β 2 -microglobulin assay ID Hs99999907_m1 (GenBank RefSeq {"type":"entrez-nucleotide","attrs":{"text":"NM_004048.2","term_id":"37704380","term_text":"NM_004048.2"}} NM_004048.2 , exon boundary 2-3; assay location, 413; amplicon size, 75 bp) ( www.ncbi.nlm.nih.gov/locuslink/refseq/ RefSeq is provided in the public domain by the National Center for Biotechnology Information, Bethesda, MD).

Techniques: Transduction, Cell Culture, Expressing, Staining, Labeling, Plasmid Preparation, Immunofluorescence

Journal: Investigative Ophthalmology & Visual Science

Article Title: Normalization of Wound Healing and Diabetic Markers in Organ Cultured Human Diabetic Corneas by Adenoviral Delivery of c-Met Gene

doi: 10.1167/iovs.09-4569

Figure Lengend Snippet: c-Met transduction did not change tight junction protein patterns. Top row: immunostaining for claudin-1 with typical membrane staining of all cell layers; bottom row: staining for ZO-1 mostly expressed in upper epithelial layers. None of these tight junction proteins appeared to change after c-met transduction. Indirect immunofluorescence of corneal sections. e, epithelium; s, stroma.

Article Snippet: The following gene-specific assay kits were used as per ABI: c-met proto-oncogene assay ID Hs00179845_m1 (GenBank RefSeq {"type":"entrez-nucleotide","attrs":{"text":"NM_001127500.1","term_id":"188595715","term_text":"NM_001127500.1"}} NM_001127500.1 , exon boundary 10-11; assay location, 2605; amplicon size, 81 bp) and β 2 -microglobulin assay ID Hs99999907_m1 (GenBank RefSeq {"type":"entrez-nucleotide","attrs":{"text":"NM_004048.2","term_id":"37704380","term_text":"NM_004048.2"}} NM_004048.2 , exon boundary 2-3; assay location, 413; amplicon size, 75 bp) ( www.ncbi.nlm.nih.gov/locuslink/refseq/ RefSeq is provided in the public domain by the National Center for Biotechnology Information, Bethesda, MD).

Techniques: Transduction, Immunostaining, Staining, Immunofluorescence

Journal: Investigative Ophthalmology & Visual Science

Article Title: Normalization of Wound Healing and Diabetic Markers in Organ Cultured Human Diabetic Corneas by Adenoviral Delivery of c-Met Gene

doi: 10.1167/iovs.09-4569

Figure Lengend Snippet: Dynamics of wound healing. A typical course of healing is presented for a c-met–transduced and vector-transduced cornea. rAV-vector–transduced diabetic cornea (top row) healed in 7 days, whereas rAV-met–transduced fellow cornea (bottom row) healed in 3 days. Pictures of live healing corneas are shown. Dashed line: shows the boundaries of the nonhealed wound region. W, wound zone; E, migrating epithelium.

Article Snippet: The following gene-specific assay kits were used as per ABI: c-met proto-oncogene assay ID Hs00179845_m1 (GenBank RefSeq {"type":"entrez-nucleotide","attrs":{"text":"NM_001127500.1","term_id":"188595715","term_text":"NM_001127500.1"}} NM_001127500.1 , exon boundary 10-11; assay location, 2605; amplicon size, 81 bp) and β 2 -microglobulin assay ID Hs99999907_m1 (GenBank RefSeq {"type":"entrez-nucleotide","attrs":{"text":"NM_004048.2","term_id":"37704380","term_text":"NM_004048.2"}} NM_004048.2 , exon boundary 2-3; assay location, 413; amplicon size, 75 bp) ( www.ncbi.nlm.nih.gov/locuslink/refseq/ RefSeq is provided in the public domain by the National Center for Biotechnology Information, Bethesda, MD).

Techniques: Plasmid Preparation

Journal: Investigative Ophthalmology & Visual Science

Article Title: Normalization of Wound Healing and Diabetic Markers in Organ Cultured Human Diabetic Corneas by Adenoviral Delivery of c-Met Gene

doi: 10.1167/iovs.09-4569

Figure Lengend Snippet: c-Met overexpression led to a significant decrease in corneal epithelial wound healing time. The average healing time of rAV-vector–transduced corneas was 6.1 days, whereas rAV-cmet–transduced corneas healed in 3.1 days on average. For comparison, previous data on normal corneas11 are also shown. They healed in 2.3 days on average. Note that vector-treated diabetic corneas healed significantly slower than normal. c-Met transduction led to a significant decrease in healing time, bringing it close to normal. Statistical analysis of the time to complete healing (n = 7 for vector and c-met, and n = 13 for normal). Significance was determined using ANOVA with the Bonferroni post test.

Article Snippet: The following gene-specific assay kits were used as per ABI: c-met proto-oncogene assay ID Hs00179845_m1 (GenBank RefSeq {"type":"entrez-nucleotide","attrs":{"text":"NM_001127500.1","term_id":"188595715","term_text":"NM_001127500.1"}} NM_001127500.1 , exon boundary 10-11; assay location, 2605; amplicon size, 81 bp) and β 2 -microglobulin assay ID Hs99999907_m1 (GenBank RefSeq {"type":"entrez-nucleotide","attrs":{"text":"NM_004048.2","term_id":"37704380","term_text":"NM_004048.2"}} NM_004048.2 , exon boundary 2-3; assay location, 413; amplicon size, 75 bp) ( www.ncbi.nlm.nih.gov/locuslink/refseq/ RefSeq is provided in the public domain by the National Center for Biotechnology Information, Bethesda, MD).

Techniques: Over Expression, Plasmid Preparation, Transduction

Journal: Investigative Ophthalmology & Visual Science

Article Title: Normalization of Wound Healing and Diabetic Markers in Organ Cultured Human Diabetic Corneas by Adenoviral Delivery of c-Met Gene

doi: 10.1167/iovs.09-4569

Figure Lengend Snippet: c-Met overexpression leads to increased phosphorylation of p38 MAP kinase. Top row: increased epithelial staining for the phosphorylated form of p38 (p-p38). Staining for phosphorylated forms of Akt (p-Akt, middle row) or ERK (p-ERK, bottom row) kinases did not show noticeable change on c-met transduction. Indirect immunofluorescence of corneal sections. e, epithelium; s, stroma.

Article Snippet: The following gene-specific assay kits were used as per ABI: c-met proto-oncogene assay ID Hs00179845_m1 (GenBank RefSeq {"type":"entrez-nucleotide","attrs":{"text":"NM_001127500.1","term_id":"188595715","term_text":"NM_001127500.1"}} NM_001127500.1 , exon boundary 10-11; assay location, 2605; amplicon size, 81 bp) and β 2 -microglobulin assay ID Hs99999907_m1 (GenBank RefSeq {"type":"entrez-nucleotide","attrs":{"text":"NM_004048.2","term_id":"37704380","term_text":"NM_004048.2"}} NM_004048.2 , exon boundary 2-3; assay location, 413; amplicon size, 75 bp) ( www.ncbi.nlm.nih.gov/locuslink/refseq/ RefSeq is provided in the public domain by the National Center for Biotechnology Information, Bethesda, MD).

Techniques: Over Expression, Staining, Transduction, Immunofluorescence

Journal: Investigative Ophthalmology & Visual Science

Article Title: Normalization of Wound Healing and Diabetic Markers in Organ Cultured Human Diabetic Corneas by Adenoviral Delivery of c-Met Gene

doi: 10.1167/iovs.09-4569

Figure Lengend Snippet: Phosphoproteomic array analysis of MAPKs. (A) On c-met transduction, phosphorylated p38α (p-p38α) was significantly (2.1-fold, n = 6, P < 0.05) increased compared with vector transduction. Array membranes were probed with total corneal protein, and signal was detected with chemiluminescence. (B) Validation of array results by Western blot analysis. Two separate cases are shown; in both of them p-p38 was increased on c-met transduction compared with vector alone. Mean increase in c-met versus vector was 1.7 ± 0.2 (n = 5, P < 0.04). Gel loading was normalized by β-actin. M, markers in kilodaltons.

Article Snippet: The following gene-specific assay kits were used as per ABI: c-met proto-oncogene assay ID Hs00179845_m1 (GenBank RefSeq {"type":"entrez-nucleotide","attrs":{"text":"NM_001127500.1","term_id":"188595715","term_text":"NM_001127500.1"}} NM_001127500.1 , exon boundary 10-11; assay location, 2605; amplicon size, 81 bp) and β 2 -microglobulin assay ID Hs99999907_m1 (GenBank RefSeq {"type":"entrez-nucleotide","attrs":{"text":"NM_004048.2","term_id":"37704380","term_text":"NM_004048.2"}} NM_004048.2 , exon boundary 2-3; assay location, 413; amplicon size, 75 bp) ( www.ncbi.nlm.nih.gov/locuslink/refseq/ RefSeq is provided in the public domain by the National Center for Biotechnology Information, Bethesda, MD).

Techniques: Transduction, Plasmid Preparation, Western Blot

Journal: Investigative Ophthalmology & Visual Science

Article Title: Normalization of Wound Healing and Diabetic Markers in Organ Cultured Human Diabetic Corneas by Adenoviral Delivery of c-Met Gene

doi: 10.1167/iovs.09-4569

Figure Lengend Snippet: Dynamics of wound healing on treatment with p38 inhibitor. (A, top row) rAV-vector–transduced diabetic cornea completely healed in 4 days in the presence of 10 μM of the inactive p38 inhibitor analogue SB 202474. Bottom row: rAV-met transduced fellow cornea healed in 6 days in the presence of 10 μM of the specific p38 inhibitor SB 202190. (B, top row) rAV-cmet–transduced diabetic cornea healed in 3 days in the presence of 10 μM SB 202474. Bottom row: rAV-met–transduced fellow cornea healed in 7 days in the presence of 10 μM SB 202190. Pictures of live corneas are shown. Analogue-treated corneas healed on average at 3.2 ± 0.4 days, compared with 5.7 ± 0.9 days for inhibitor-treated corneas (P < 0.05). p38 inhibitor thus abrogates c-met–induced acceleration of epithelial wound healing. Dashed line: shows the boundaries of the nonhealed wound region. W, wound zone; E, migrating epithelium.

Article Snippet: The following gene-specific assay kits were used as per ABI: c-met proto-oncogene assay ID Hs00179845_m1 (GenBank RefSeq {"type":"entrez-nucleotide","attrs":{"text":"NM_001127500.1","term_id":"188595715","term_text":"NM_001127500.1"}} NM_001127500.1 , exon boundary 10-11; assay location, 2605; amplicon size, 81 bp) and β 2 -microglobulin assay ID Hs99999907_m1 (GenBank RefSeq {"type":"entrez-nucleotide","attrs":{"text":"NM_004048.2","term_id":"37704380","term_text":"NM_004048.2"}} NM_004048.2 , exon boundary 2-3; assay location, 413; amplicon size, 75 bp) ( www.ncbi.nlm.nih.gov/locuslink/refseq/ RefSeq is provided in the public domain by the National Center for Biotechnology Information, Bethesda, MD).

Techniques: Plasmid Preparation

Journal: Genes to Cells

Article Title: Generation of metabolically functional hepatocyte‐like cells from dedifferentiated fat cells by Foxa2, Hnf4a and Sall1 transduction

doi: 10.1111/gtc.12814

Figure Lengend Snippet: Hepatic functions in FHS‐DFAT cells before and after hepatic induction. (a) FHS‐DFAT cells were cultured in hepatic medium for 14 days. The temporal expressions of hepatocyte marker genes Afp, Alb, Tat and Tdo2 were examined by real‐time PCR analyses in FHS‐DFAT cells during hepatic differentiation. Mean values ± SEM . were normalized to Gapdh and expressed relative to normal hepatocytes from adult mice. p ‐Value was calculated using Tukey's honestly significant difference test (a–d: p < .01). (b) FHS‐DFAT cells and GFP‐DFAT cells (Ctrl) before (D0) and after (D14) induction of differentiation and HCs were subjected to immunostaining with anti‐Alb (red) antibodies. Nuclei were stained with Hoechst 33342 (blue). (c) The Alb secretion capacity was examined by ELISA. ND, not detected. (d) Glycogen stores were showed by PAS staining. (e) Total cellular glycogen of FHS‐DFAT cells and HCs was examined. Superscript letters indicate significant ( p < .01) differences. p ‐Value was calculated using Tukey's honestly significant difference test. ND, not detected. (f) FHS‐DFAT cells and HCs were cultured in a medium containing LDL (red) for 4 hr, and immunohistochemistry was carried out. Nuclei were counterstained with Hoechst 33342 (blue). All scale bars represent 50 μm. All quantitative data are means ± SD . ( n = 3 experiments)

Article Snippet: The probes for Alb (GenBank accession no. NM_009654.3; Mm00802090_m1), Afp (GenBank accession no. NM_007423.4; Mm00431715_m1), Tat (GenBank accession no. NM_146214.3; Mm01244282_m1), Tdo2 (GenBank accession no. NM_019911.2; Mm00451269_m1), Cyp1a2 (GenBank accession no. NM_009993.3; Mm00487224_m1), Cyp2e1 (GenBank accession no. NM_021282.2; Mm00491127_m1), Cyp2a5 (GenBank accession no. NM_007812.4; Mm00487248_g1), Cyp7a1 (GenBank accession no. NM_007824.2; Mm00484150_m1), Lgr5 (GenBank accession no. NM_010195.2; Mm00438890_m1), Otc (GenBank accession no. NM_008769.3; Mm00493267_m1) and Pck1 (GenBank accession no. NM_011044.2; Mm01247058_m1) genes were obtained from TaqMan Pre‐Developed Assay Reagents (Applied Biosystems).

Techniques: Cell Culture, Marker, Real-time Polymerase Chain Reaction, Immunostaining, Staining, Enzyme-linked Immunosorbent Assay, Immunohistochemistry

Journal: Nature

Article Title: Specification of claustro-amygdalar and palaeocortical neurons and circuits.

doi: 10.1038/s41586-024-08361-5

Figure Lengend Snippet: Fig. 1 | Regulation of VLp development and Tfap2d expression by SOX4 and SOX11. a, Coronal sections from VLp of mouse brains from wild-type (WT), Sox4-cKO, Sox11-cKO and Sox4/Sox11-cdKO mice. Nissl staining highlights the BLC. In situ hybridization for Lmo3, Mef2c and Etv1 marks BLC, LA and BLA, whereas GFP and SATB1 immunostaining labels BLC. White and black filled arrowheads highlight normal phenotypes and open arrowheads highlight defective phenotypes. Scale bar, 200 μm. b, Intersection of differentially expressed genes in Sox4-cKO, Sox11-cKO and Sox4/Sox11-cdKO cortex and amygdala compared with controls (n = 3 per genotype). c, Top 50 genes with decreased expression in Sox4/Sox11-cdKO samples over Sox4-cKO, Sox11-cKO and control samples. FC, fold change. d, In situ hybridization for Tfap2d in the VLp of control, Sox4-cKO, Sox11-cKO and Sox4/Sox11-cdKO brains. Scale bars, 500 μm. e, Quantitative PCR showing the percentage of input of six potential Tfap2d enhancers (E1–E6) after ChIP on PCD15.5 wild-type and Sox11-cKO cortices,

Article Snippet: GFP (1:500; Abcam, ab13970; Chicken polyclonal IgY, IHC, Kaur etal, PMID: 32707082) RFP (1:500, Abcam, ab124754; Kaur etal, PMID: 32707082) anti-cleaved capase3 (1:500; Cell Signaling, Cat. 9611, rabbit polyclonal IgG, IHC, Shim et al. 2012 (PMID: 22678282)) anti-SOX11 (Abcam, ab229185, 1080158-1), PolII (Sigma-Aldrich, 05-623),IgG (Diagenode, C15410206) ADGRE1 (1:500, Biorad, MCA497RT, Shi et al., PMID: 31197172), Alexa Fluor® 594 AffiniPure Donkey Anti-Goat IgG,1:250- 1:500,Jackson ImmunoResearch, Catalog No. 705-585-147, Lot No.169491 Alexa Fluor® 594 AffiniPure Donkey Anti-Rabbit IgG, 1:250- 1:500, Jackson ImmunoResearch, Catalog No.711-585-152, Lot No.171656 Alexa Fluor® 647 AffiniPure Donkey Anti-Mouse IgG, 1:250- 1:500, Jackson ImmunoResearch, Catalog No.715-605-151, Lot No.168259 Alexa Fluor® 594 AffiniPure Donkey Anti-Mouse IgG, 1:250- 1:500, Jackson ImmunoResearch, Catalog No.715-585-150, Lot No.167435 Alexa Fluor® 488 AffiniPure Donkey Anti-Chicken IgY, 1:250- 1:500, Jackson ImmunoResearch, Catalog No.703-545-155, Lot No.168728 Alexa Fluor® 594 AffiniPure Donkey Anti-Goat IgG (H+L), 1:250- 1:500, Jackson ImmunoResearch, Catalog No. 705-585-003 Alexa Fluor® 647 AffiniPureTM Donkey Anti-Goat IgG (H+L), 1:250- 1:500, Jackson ImmunoResearch, Catalog No.705-605-003 Donkey anti mouse - 594, 1:250- 1:500, Jackson ImmunoResearch, Catalog No.715-585-150 Alexa Fluor® 594 AffiniPureTM Goat Anti-Rabbit IgG (H+L), 1:250- 1:500, Jackson Immunoresearch, Catalog No.111-585-003 Alexa Fluor® 647 AffiniPureTM Donkey Anti-Goat IgG (H+L), 1:250- 1:500, Jackson ImmunoResearch, Catalog No.705-605-003, Lot No.705605003 Validation SOX11 antibody was validated using the SOX11 KO mice ( Figure 1e, Extended data figure 2) Eukaryotic cell lines Policy information about cell lines and Sex and Gender in Research Cell line source(s) Neuro2a mouse neuroblastoma cell line was purchased from ATCC.

Techniques: Expressing, Staining, In Situ Hybridization, Immunostaining, Control, Real-time Polymerase Chain Reaction